Immobilization of Alpha-amylase and glucoamylase to sepharose - 4B
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چکیده
منابع مشابه
Coupling of glycosaminoglycans to agarose beads (sepharose 4B).
1. Heparin, heparan sulphate, chondroitin sulphate and dermatan sulphate were covalently attached to beads of agarose activated by cyanogen bromide. The bond is probably mediated by the amino group of a serine or peptide residue at the reducing end of the polysaccharide chain. 2. The uptake of glycosaminoglycan during the coupling procedure is about 0.9mg/ml of wet gel. However, direct analysis...
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Human salivary amylase and pancreatic amylase were purified and characterized. These amylases gave two bands and one band, respectively, each staining for both protein and sugar, after electrophoresis on sodium dodecyl sulfate--polyacrylamide gel. The relative molecular mass (Mr) or pancreatic amylase was calculated to be 60 000; for the two components (A and B) of salivary amylase the Mr were ...
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ژورنال
عنوان ژورنال: Journal of the National Science Foundation of Sri Lanka
سال: 1987
ISSN: 2362-0161,1391-4588
DOI: 10.4038/jnsfsr.v15i2.8296